Fig. 2. Characteristics of mitochondrial morphology and mass of WT and VDAC1^-/- H9c2 cells. (A) Immunostaining of WT and VDAC1^-/-C1 H9c2 cells with COX IV antibody. (B) Histogram shows average values of two mitochondrial shape metrics, aspect ratio (left panel) and form factor (right panel) from 20 cells/group. *^{, #}P<0.05 vs. WT. (C) Histogram shows relative ratio of mtDNA Ct/β-actin DNA Ct detected by real time PCR from WT and VDAC1^-/-C1, C2 and C3 H9c2 cells. * P<0.05 vs. WT.